Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis.
- Author:
Soo Jin CHO
1
;
Young Hwan KIM
;
Hee Moon PARK
;
Kwang Soo SHIN
Author Information
1. BioRefinery Center, Korea Research Institute of Chemical Technology, Daejeon 305-600, Korea.
- Publication Type:Original Article
- Keywords:
Lkh1;
Proteome analyses;
Schizosaccharomyces pombe
- MeSH:
Acrylic Resins;
beta-Glucosidase;
Cell Wall;
Databases, Protein;
Electrophoresis, Polyacrylamide Gel;
Glucan 1,3-beta-Glucosidase;
Phosphotransferases;
Proteins;
Proteome;
Schizosaccharomyces;
Tandem Mass Spectrometry
- From:Mycobiology
2010;38(2):108-112
- CountryRepublic of Korea
- Language:English
-
Abstract:
To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. Differentially expressed proteins were identified by tandem mass spectrometry (MS/MS) and were compared with a protein database. In whole-cell extracts, 10 proteins were up-regulated and 9 proteins were down-regulated in the mutant. In extracellular preparations, 6 proteins were up-regulated in the lkh1 + null mutant and 4 proteins successfully identified: glycolipid anchored surface precursor, beta-glucosidase (Psu1), cell surface protein, glucan 1,3-beta-glucosidase (Bgl2), and exo-1,3 beta-glucanase (Exg1). These results suggest that Lkh1 is involved in regulating cell wall assembly.
