Molecular Analysis of Exophiala Species Using Molecular Markers.
- Author:
Hee Youn CHEE
1
;
Yoon Kyoung KIM
Author Information
1. Division of Biological Sciences, Medical School, Konyang University, Nonsan, Chungnam 320-711, Korea.
- Publication Type:Original Article
- Keywords:
Exophiala;
Genetic relatedness;
(GTG)5;
M-13;
PCR-RFLP;
Ribosomal DNA
- MeSH:
Base Sequence;
DNA, Ribosomal;
Exophiala*;
Genetic Markers;
Nucleotides;
Polymorphism, Restriction Fragment Length
- From:Mycobiology
2002;30(1):1-4
- CountryRepublic of Korea
- Language:English
-
Abstract:
Genetic relatedness of medically important Exophiala species such as E. dermatitidis, E. mansonii, and three E. jeanselmei varieties: jeanselmei, lecanii-corni, and heteromorpha was examined using PCR-RFLP (restriction fragment length polymorphism) of ribosomal DNA, M-13, (GTG)5, and nucleotide sequences of ribosomal ITS (internal transcribed space) II regions. Three E. jeanselmei varieties showing distinct band patterns for each DNA markers as well as different nucleotide sequences of ribosomal ITS II regions could be considered as a separate species. E. dermatitidis and E. mansonii demonstrated the identical band patterns of RFLP of ribosomal DNA, M-13, and (GTG)5 markers. However, nucleotides sequences of ribosomal ITS II region were different between these two species.
