Sequence Analyses of PCR Amplified Partial SSU of Ribosomal DNA for Identifying Arbuscular Mycorrhizal Fungi in Plant Roots.
- Author:
Moon Sung TAE
1
;
Ahn Heum EOM
;
Sang Sun LEE
Author Information
1. Department of Biology, Korea National University of Education, Chungbuk 363-791, Republic of Korea. sslee@cc.knue.ac.kr
- Publication Type:Original Article
- Keywords:
Arbuscular mycorrhizal fungi;
Glycine max;
PCR-RFLP;
Sorghum bicolor
- MeSH:
Colon;
DNA;
DNA, Fungal;
DNA, Ribosomal*;
Fungi*;
Genes, rRNA;
Plant Roots*;
Plants*;
Polymerase Chain Reaction*;
Sequence Analysis*;
Sorghum;
Soybeans;
Spores
- From:Mycobiology
2002;30(1):13-17
- CountryRepublic of Korea
- Language:English
-
Abstract:
The genomic DNAs were extracted from roots of Glycine max and Sorghum bicolor, and compared with those from spores of two arbuscular mycorrhizal (AM) fungi, Glomus mosseae and Scutellospora heterogama. The partial small subunit (SSU) of ribosomal RNA genes were synthesized and amplified by polymerase chain reaction with the fungal specific primers, AM1 and NS31. By the recent molecular techniques, the presence of another AM fungal DNA were confirmed in the roots of two plants, and three sequences of rDNA fragments amplified were identified to be close to those of G. caledonium, G. fasiculatum and G. proliferum. The two AM fungi, both, were found to colonize at the cortical layers of plant roots collected in the fields, together.
