Optimization of Solid-State Fermentation for Improved Conidia Production of Beauveria bassiana as a Mycoinsecticide.
- Author:
Tuan Anh PHAM
1
;
Jeong Jun KIM
;
Keun KIM
Author Information
1. Department of Bioscience and Biotechnology, The University of Suwon, Hwaseong 445-743, Korea. kkim@suwon.ac.kr
- Publication Type:Original Article
- Keywords:
Beauveria bassiana;
Conidia production;
Optimal culture conditions;
Solid-state fermentation
- MeSH:
Aphids;
Beauveria;
Fermentation;
Humans;
Meals;
Polyethylene;
Seeds;
Spores, Fungal;
Zea mays
- From:Mycobiology
2010;38(2):137-143
- CountryRepublic of Korea
- Language:English
-
Abstract:
The production of conidia of entomopathogenic Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent against aphid Myzus persicae. The optimal conditions for conidia production on polished white rice were 40% moisture content, 25degrees C culture temperature, 2-day-old seeding culture grown in 3% corn meal, 2% rice bran, 2% corn steep powder medium, initial conidia concentration of 107 conidia/g in the wet rice, 10% inoculum size, and use of a polyethylene bag as a container. The polyethylene bag containing inoculated rice was hand-shaken every 12 hr during fermentation. Using optimal conditions, the maximum conidia production obtained was 4.05 g conidia/100 g dry rice after 14 days of cultivation, a rate 2.83 times higher than conidia yield of pre-optimization.
