Chinese Cabbage Clubroot Pathogen, Plasmodiophora brassicae, Is Genetically Stable.
10.4489/MYCO.2009.37.3.225
- Author:
Seung Hwan HEO
1
;
Se Jeong JANG
;
Jin Soo CHOI
;
Chang Soon JANG
;
Jeong Young SONG
;
Hong Gi KIM
Author Information
1. National Plant Quarantine Service, Gunsan 573-879, Korea.
- Publication Type:Original Article
- Keywords:
Genetic variation;
Plasmodiophora brassicae;
Progeny analysis;
RAPD;
Single spore isolate
- MeSH:
Asian Continental Ancestry Group;
Brassica;
Continental Population Groups;
DNA;
Genetic Variation;
Humans;
Plasmodiophorida;
Spores
- From:Mycobiology
2009;37(3):225-229
- CountryRepublic of Korea
- Language:English
-
Abstract:
Single spore isolates of Plasmodiophora brassicae e4 and e9 obtained from diseased Chinese cabbage were identified as race 4 and race 9, respectively, by the Williams' differential variety set. To confirm the possibility of variation in same generation and progeny of a single spore isolate of P. brassicae, random amplified polymorphic DNA (RAPD) analysis was conducted using the URP 3, 6 and OPA 7 primers. There was no difference in band type at each part of the gall of Chinese cabbage obtained by inoculation of e4 and e9 and amplification using the URP 3 and 6 primers when the same generation was analyzed. In addition, the progeny analysis, which was expanded to the third generation and conducted using the URP 3 and OPA 7 primers, revealed no differences in the band type of the e4 isolate. Based on these results, the single spore isolate of P. brassicae was genetically stable.
