Isolation of Genes Specifically Expressed in Different Developmental Stages of Pleurotus ostreatus Using Macroarray Analysis.
10.4489/MYCO.2009.37.3.230
- Author:
Seung Ho LEE
1
;
Joong Ho JOH
;
Jin Sung LEE
;
Jong Hyun LIM
;
Kyung Yun KIM
;
Young Bok YOO
;
Chang Soo LEE
;
Beom Gi KIM
Author Information
1. Bio-crops Development Division, National Academy of Agricultural Science, RDA, Suwon, Korea. bgkimpeace@gmail.com
- Publication Type:Original Article
- Keywords:
Pleurotus ostreatus;
Fruiting body development;
Oyster mushroom;
Specific gene expression
- MeSH:
Agaricales;
Blotting, Northern;
Breeding;
Clone Cells;
Estrone;
Expressed Sequence Tags;
Fruit;
Mycelium;
Pleurotus;
Publications;
Transcriptome
- From:Mycobiology
2009;37(3):230-237
- CountryRepublic of Korea
- Language:English
-
Abstract:
The oyster mushroom (Pleurotus ostreatus) is one of the most important edible mushrooms worldwide. The mechanism of P. ostreatus fruiting body development has been of interest both for the basic understanding of the phenotypic change of the mycelium-fruiting body and to improve breeding of the mushrooms. Based on our previous publication of P. ostreatus expressed sequence tag database, 1,528 unigene clones were used in macroarray analysis of mycelium, fruiting body and basidiospore developmental stages of P. ostreatus. Gene expression profile databases generated by evaluating expression levels showed that 33, 10, and 94 genes were abundantly expressed in mycelium, fruiting body and basidiospore developmental stages, respectively. Among them, the genes specifically expressed in the fruiting body stage were further analyzed by reverse transcription-polymerase chain reaction and Northern blot to investigate temporal and spatial expression patterns. These results provide useful information for future studies of edible mushroom development.
