An Efficient Method to Prepare PCR Cloning Vectors.
10.4489/MYCO.2009.37.3.240
- Author:
Soon Gyu HONG
1
;
Ji Young CHOI
;
Barry M PRYOR
;
Hong Kum LEE
Author Information
1. Polar BioCenter, Korea Polar Research Institute, KORDI, Songdo Techno Park, Songdo-dong 7-50, Yeonsu-gu, Incheon 406-840, Korea. polypore@kopri.re.kr
- Publication Type:Brief Communication
- Keywords:
PCR cloning vector;
XcmI
- MeSH:
Clone Cells;
Cloning, Organism;
Digestion;
Genetic Vectors;
Ligation;
Polymerase Chain Reaction
- From:Mycobiology
2009;37(3):240-242
- CountryRepublic of Korea
- Language:English
-
Abstract:
An improved procedure for preparing PCR cloning vectors was developed. This procedure includes the incorporation of adapters to create XcmI restriction enzyme sites in pBluescript II SK(+) vectors, digestion with XcmI followed by further digestion of the small fragment produced by XcmI digestion with additional enzymes, and purification with PCR purification kits. Using this procedure, PCR cloning vectors with high ligation efficiencies and low blue or false-positive colonies were obtained.
