Optimal Medium Conditions for the Detection of Cellulolytic Activity in Ganoderma lucidum.
10.4489/MYCO.2009.37.4.313
- Author:
Woo Sik JO
1
;
Soon Hwa BAE
;
Doo Hyun CHO
;
So Deuk PARK
;
Young Bok YOO
;
Seung Chun PARK
Author Information
1. Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-320, Korea. jowoosik@yahoo.co.kr
- Publication Type:Brief Communication
- Keywords:
Cellulolytic enzyme;
Chromogenic media;
Congo red;
Ganoderma lucidum
- MeSH:
Benzenesulfonates;
Coloring Agents;
Congo Red;
Diminazene;
Fungi;
Ganoderma;
Hydrogen-Ion Concentration;
Phenolsulfonphthalein;
Reishi;
Trypan Blue
- From:Mycobiology
2009;37(4):313-316
- CountryRepublic of Korea
- Language:English
-
Abstract:
To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to 35degrees C. On the whole, the best protocol called for Ganoderma lucidum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at 25degrees C for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma lucidum.
