Development of Specific Primer for Tricholoma matsutake.
10.4489/MYCO.2009.37.4.317
- Author:
Jang Han KIM
1
;
Yeong Hwan HAN
Author Information
1. Department of Life Science, Graduate School, Dongguk University, Gyeongju, Gyeongbuk 780-714, Korea.
- Publication Type:Brief Communication
- Keywords:
ITS;
Tricholoma matsutake;
Tricholomataceae
- MeSH:
Korea;
Polymerase Chain Reaction;
Tricholoma
- From:Mycobiology
2009;37(4):317-319
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'-GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.
