Generation and Evaluation of High beta-Glucan Producing Mutant Strains of Sparassis crispa.
10.5941/MYCO.2013.41.3.159
- Author:
Seung Rak KIM
1
;
Hyeon Woo KANG
;
Hyeon Su RO
Author Information
1. Department of Microbiology and Research Institute of Life Sciences, Gyeongsang National University, Jinju 660-701, Korea. rohyeon@gnu.ac.kr
- Publication Type:Brief Communication
- Keywords:
Beta-glucan;
Chemical mutagenesis;
Growth rate;
Sparassis crispa
- MeSH:
Agaricales;
Efficiency;
Mass Screening;
Mesylates;
Methyl Methanesulfonate;
Mutagenesis;
Sprains and Strains
- From:Mycobiology
2013;41(3):159-163
- CountryRepublic of Korea
- Language:English
-
Abstract:
A chemical mutagenesis technique was employed for development of mutant strains of Sparassis crispa targeting the shortened cultivation time and the high beta-glucan content. The homogenized mycelial fragments of S. crispa IUM4010 strain were treated with 0.2 vol% methyl methanesulfonate, an alkylating agent, yielding 199 mutant strains. Subsequent screening in terms of growth and beta-glucan content yielded two mutant strains, B4 and S7. Both mutants exhibited a significant increase in beta-glucan productivity by producing 0.254 and 0.236 mg soluble beta-glucan/mg dry cell weight for the B4 and S7 strains, respectively, whereas the wild type strain produced 0.102 mg soluble beta-glucan/mg dry cell weight. The results demonstrate the usefulness of chemical mutagenesis for generation of mutant mushroom strains.
