Effects of Age on in vitro Maturation and Fertilization of Immature Oocytes from Stimulated Cycles in Human IVF-ET Program.
- Author:
Sang Hoon HAN
;
Jung Ryeol LEE
;
Hyun Jun KIM
;
Jung Hee MOON
;
Byung Chul JEE
;
Seung Yup KU
;
Chang Suk SUH
;
Seok Hyun KIM
;
Young Min CHOI
;
Jung Gu KIM
;
Shin Yong MOON
- Publication Type:In Vitro ; Original Article
- Keywords:
Immature oocyte;
Female age;
In vitro maturation;
Controlled ovarian hyperstimulation;
Fertilization
- MeSH:
Adult;
Epidermal Growth Factor;
Female;
Fertilization*;
Follicular Fluid;
Humans*;
Metaphase;
Oocytes*;
Ovulation;
Sperm Injections, Intracytoplasmic
- From:Korean Journal of Fertility and Sterility
2005;32(4):331-336
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the effects of female age on in vitro maturation and fertilization of immature oocytes from controlled ovarian hyperstimulation (COH) in human IVF-ET program. METHOD: A total of 96 immature oocytes (GV & metaphase I) obtained from 40 cycles of IVF-ET (29 patients). The mean age of female patients was 31.8+/-3.1 years. Ovulation was triggered by urinary or recombinant hCG. Immature oocytes were cultured with YS medium containing 30% of patients' human follicular fluids, LH (1 IU/mL), FSH (1 IU/mL) and EGF (10 ng/mL), and then matured oocytes were fertilized by ICSI. In vitro maturation and fertilization of immature oocytes were analyzed according to age of female (< 34 or > or = 34 years). RESULTS: The maturation rate was similar between two groups (68% vs 64%). The fertilization rate of in?vitro-matured oocytes was higher in patients < 34 years old, but there was no statistical significance (64% vs 50%, p=0.347). The fertilization rate of in-vitro-matured oocytes was significantly lower compared with those of in-vivo-matured oocytes in both age groups (64% vs 79%, p=0.035, 50% vs 86%, p=0.007). CONCLUSION: In older female group, fertilization rate of in-vitro-matured oocytes seems to be decreased. Further investigations should be warranted to increase fertilization potential of in-vitro-matured oocytes.
