Trichoderma asperellum Chi42 Genes Encode Chitinase.
- Author:
Nguyen Hoang LOC
1
;
Hoang Tan QUANG
;
Nguyen Bao HUNG
;
Nguyen Duc HUY
;
Truong Thi Bich PHUONG
;
Tran Thi Thu HA
Author Information
1. Institute of Resources, Environment and Biotechnology, Hue University, 27 Phan Dinh Phung St., Hue, Vietnam. nhloc@hueuni.edu.vn
- Publication Type:Original Article
- Keywords:
Chi42 gene;
Internal transcribed spacer region;
Trichoderma asperellum;
42 kDa chitinase
- MeSH:
Agar;
Amino Acid Sequence;
Chitin;
Chitinase;
Colloids;
Molecular Weight;
Soil;
Sprains and Strains;
Trichoderma;
Vietnam
- From:Mycobiology
2011;39(3):182-186
- CountryRepublic of Korea
- Language:English
-
Abstract:
Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.
