Genes Expressed During Fruiting Body Formation of Agrocybe cylindracea.
- Author:
Sung Mi SHIM
1
;
Sang Beom KIM
;
Hey Young KIM
;
Hyun Su RHO
;
Hyun Sook LEE
;
Min Woong LEE
;
U Youn LEE
;
Kyung Hoan IM
;
Tae Soo LEE
Author Information
1. Department of Biology, University of Incheon, Incheon 402-749, Korea. tslee@incheon.ac.kr
- Publication Type:Original Article
- Keywords:
Agrocybe cylindracea;
cDNA clones;
Differential screening;
Gene expression
- MeSH:
Agaricales;
Agrocybe*;
Amino Acid Transport Systems;
Clone Cells;
Cytochrome P-450 Enzyme System;
Cytotoxins;
DNA, Complementary;
Fruit*;
Gene Expression;
Morphogenesis;
Peptidylprolyl Isomerase;
Perforin;
Serine Proteases
- From:Mycobiology
2006;34(4):209-213
- CountryRepublic of Korea
- Language:English
-
Abstract:
Agrocybe cylindracea, an edible mushroom belonging to Bolbitiaceae, Agaricales, is widely used as invaluable medicinal material in the oriental countries. This study was initiated to find the genes expressed during the fruiting body formation of A. cylindracea. The cDNAs expressed differentially during fruiting body morphogenesis of A. cylindracea were isolated through subtractive hybridization between vegetative mycelia and fruiting bodies. The cDNAs expressed in the fruiting body morphogenesis of A. cylindracea were cloned and twenty genes were identified. Eleven were homologous to genes of known functions, three were homologous to genes in other organism without any function known. Six were completely novel genes specific to A. cylindracea so far examined. Some genes with known functions were a pleurotolysin, a self-assembling poreforming cytolysins; Aa-Pri1 and Pir2p, specifically induced genes during fruiting initiation of other mushroom, Agrocybe aegerita; an amino acid permease; a cytochrome P450; a MADS-box gene; a peptidylprolyl isomerase; and a serine proteinase. For other clones, no clear function was annotated so far. We believe the first report of the differentially expressed genes in fruiting process of A. cylindracea will be great helps for further research.
