PCR-Based Sensitive Detection of Wood-Decaying Fungus Phellinus linteus by Specific Primer from rDNA ITS Regions.
- Author:
Dong Suk PARK
;
Hee Wan KANG
;
Ki Tae KIM
;
Soo Muk CHO
;
Young Jin PARK
;
Hye Sun SHIN
;
Byoung Moo LEE
;
Seung Joo GO
- Publication Type:Original Article
- Keywords:
Phellinus linteus;
rDNA;
Primer;
Detection
- MeSH:
DNA, Ribosomal*;
Fungi*;
Polymerase Chain Reaction
- From:Mycobiology
2001;29(1):7-10
- CountryRepublic of Korea
- Language:English
-
Abstract:
Based on the rDNA ITS sequences data, specific primer set for PCR detection of wood-decaying fungus Phellinus linteus was designed. The length of PCR products using designed primer set(SHF and SHR) was about 540 bp. Among 11 species, 17 isolates of Phellinus spp. including Phellinus linteus, P. pomaceus, P. spiculosus, P. baumi, P. pini, P. igniarius, P. gilvus, P. biscuspidatus, P. weirii, P. johnsonianus, P. robutus, and P. igniarius, seven isolates of Phellinus linteus showed about 540 bp-sized single band. This molecular technique could offer a useful tool for detecting and identifying Phellinus linteus.
