Phylogenetic Placement and Morphological Characterization of Sclerotium rolfsii (Teleomorph: Athelia rolfsii) Associated with Blight Disease of Ipomoea batatas in Korea.
10.5941/MYCO.2017.45.3.129
- Author:
Narayan Chandra PAUL
1
;
Eom Ji HWANG
;
Sang Sik NAM
;
Hyeong Un LEE
;
Joon Seol LEE
;
Gyeong Dan YU
;
Yong Gu KANG
;
Kyeong Bo LEE
;
San GO
;
Jung Wook YANG
Author Information
1. Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration, Muan 58545, Korea. hg369732@korea.kr
- Publication Type:Original Article
- Keywords:
Fungal morphology;
Ipomoea batatas;
Molecular phylogeny;
Pathogenicity;
Sclerotium rolfsii
- MeSH:
Agriculture;
Fungi;
Humans;
Incidence;
Ipomoea batatas*;
Ipomoea*;
Korea*;
Mothers;
Soil;
Virulence
- From:Mycobiology
2017;45(3):129-138
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, we aimed to characterize fungal samples from necrotic lesions on collar regions observed in different sweetpotato growing regions during 2015 and 2016 in Korea. Sclerotia appeared on the root zone soil surface, and white dense mycelia were observed. At the later stages of infection, mother roots quickly rotted, and large areas of the plants were destroyed. The disease occurrence was monitored at 45 and 84 farms, and 11.8% and 6.8% of the land areas were found to be infected in 2015 and 2016, respectively. Fungi were isolated from disease samples, and 36 strains were preserved. Based on the cultural and morphological characteristics of colonies, the isolates resembled the reference strain of Sclerotium rolfsii. Representative strains were identified as S. rolfsii (teleomorph: Athelia rolfsii) based on phylogenetic analysis of the internal transcribed spacer and large subunit genes along with morphological observations. To test the pathogenicity, sweetpotato storage roots were inoculated with different S. rolfsii strains. ‘Yulmi’ variety displayed the highest disease incidence, whereas ‘Pungwonmi’ resulted in the least. These findings suggested that morphological characteristics and molecular phylogenetic analysis were useful for identification of S. rolfsii.
