Assessment of Collagen-Induced Arthritis Using Cyanine 5.5 Conjugated with Hydrophobically Modified Glycol Chitosan Nanoparticles: Correlation with 18F-Fluorodeoxyglucose Positron Emission Tomography Data.
10.3348/kjr.2012.13.4.450
- Author:
Ji Hyeon CHA
1
;
Sang Hoon LEE
;
Sheen Woo LEE
;
Kyeongsoon PARK
;
Dae Hyuk MOON
;
Kwangmeyung KIM
;
Sandip BISWAL
Author Information
1. Department of Radiology and Research Institute of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Korea. shlee@amc.seoul.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
HGC-Cy5.5;
18F-FDG PET;
Near-infrared fluorescence imaging;
Rheumatoid arthritis
- MeSH:
Animals;
Ankle Joint/radionuclide imaging;
Arthritis, Experimental/*radionuclide imaging;
Carbocyanines/administration & dosage/*diagnostic use;
Chitosan/administration & dosage/*diagnostic use;
Fluorodeoxyglucose F18/administration & dosage/diagnostic use;
Injections, Intravenous;
Knee Joint/radionuclide imaging;
Male;
Mice;
Microscopy, Confocal;
Nanoparticles;
Positron-Emission Tomography/*methods;
Radiopharmaceuticals/administration & dosage/diagnostic use;
Statistics, Nonparametric
- From:Korean Journal of Radiology
2012;13(4):450-457
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To evaluate the potential and correlation between near-infrared fluorescence (NIRF) imaging using cyanine 5.5 conjugated with hydrophobically modified glycol chitosan nanoparticles (HGC-Cy5.5) and 18F-fluorodeoxyglucose-positron emission tomography (18F-FDG-PET) imaging of collagen-induced arthritis (CIA). MATERIALS AND METHODS: We used 10 CIA and 3 normal mice. Nine days after the injecting collagen twice, microPET imaging was performed 40 minutes after the intravenous injection of 9.3 MBq 18F-FDG in 200 microL PBS. One day later, NIRF imaging was performed two hours after the intravenous injection of HGC-cy5.5 (5 mg/kg). We assessed the correlation between these two modalities in the knees and ankles of CIA mice. RESULTS: The mean standardized uptake values of 18F-FDG for knees and ankles were 1.68 +/- 0.76 and 0.79 +/- 0.71, respectively, for CIA mice; and 0.57 +/- 0.17 and 0.54 +/- 0.20 respectively for control mice. From the NIRF images, the total photon counts per 30 mm2 for knees and ankles were 2.32 +/- 1.54 x 10(5) and 2.75 +/- 1.51 x 10(5), respectively, for CIA mice, and 1.22 +/- 0.27 x 10(5) and 0.88 +/- 0.24 x 10(5), respectively, for control mice. These two modalities showed a moderate correlation for knees (r = 0.604, p = 0.005) and ankles (r = 0.464, p = 0.039). Moreover, both HGC-Cy5.5 (p = 0.002) and 18F-FDG-PET (p = 0.005) imaging also showed statistically significant differences between CIA and normal mice. CONCLUSION: NIRF imaging using HGC-Cy5.5 was moderately correlated with 18F-FDG-PET imaging in the CIA model. As such, HGC-Cy5.5 imaging can be used for the early detection of rheumatoid arthritis.
