Several Genes Expressed During Morphogenesis of Lentinus edodes (ImHyup-1).
- Author:
Sang Sun LEE
1
;
Sung Woon HONG
;
Seung Hae KIM
;
Bong Cheol KIM
Author Information
1. Major in Biological Science and Education, Graduate School, Korea National University of Education, Chungju, Chungpuk 360-791, Korea. sslee@cc.knue.ac.kr
- Publication Type:Original Article
- Keywords:
DDRT-PCR;
Lentinus edodes;
Morphogenesis
- MeSH:
Clone Cells;
DNA, Complementary;
Fruit;
Lentinula*;
Morphogenesis*;
Reverse Transcription;
RNA;
Sequence Analysis, DNA;
Shiitake Mushrooms*
- From:Mycobiology
2001;29(3):135-141
- CountryRepublic of Korea
- Language:English
-
Abstract:
Differential display of reverse transcription (DDRT)-PCR was conducted to have a profile of the differentially expressed genes during the formation of fruiting body of Lentinus edodes. The lines of L. edodes (ImHyup-1) employed were cultivated in the artificial blocks of sawdust, and the fruiting body was induced from the mycelia or the mass protruded from the brown surface of the sawdust blocks. RNAs were prepared from the four different developmental stages; mycelial, primordial, and stipes and pileus of fruiting body. The fragments of cDNA were synthesized from the combinations of the arbitrary primers and 3' one anchored Oligo-dT primer. Twelve combinations using the primers have been tested, and among them nineteen bands were identified as differentially expressed. Those genes were further analyzed by DNA sequencing and followed by homology search. Characterization of one clone was conducted as a preliminary data and more are under investigation.
