Availability of Sikhae Factory Wastewater as a Submerged Culture Medium for Lentinula edodes.
- Author:
Heon Ho JUNG
1
;
Jae Yun LEE
;
Gi Young KIM
;
Hyung Sik PARK
;
Byung Hyouk NAM
;
Won Gun AN
;
Sang Joon LEE
;
Jae Dong LEE
Author Information
1. Department of Microbiology, College of Natural Sciences, Pusan National University, Pusan 609-735, Korea. leejd@hyowon.pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Lentinula edodes;
Submerged culture;
Sikhae factory wastewater
- MeSH:
Beverages;
Edible Grain;
Fruit;
Hydrogen-Ion Concentration;
Lentinula*;
Shiitake Mushrooms*;
Waste Water*
- From:Mycobiology
2001;29(3):160-163
- CountryRepublic of Korea
- Language:English
-
Abstract:
Sikhae is a Korean traditional beverage of saccharified rice. Its factory waste (SFW) is usually thrown away instead of being used. We developed a cheap substrate of SFW for use in liquid spawn that is known for its higher fruit body yields than grain spawn in sawdust cultivation. Mycelia of Lentinula edodes ASI 3046, which is regarded as the most suitable strain for sawdust cultivation, were cultured on six kinds of previous known media and SFW. As the seven kinds of media were applied, a Sikhae Factory Waste (SFW) was most excellent in growth. The dried mycelial weight in SFW was almost four times as much as that in the other media. In the flask culture, optimum culture conditions for the mycelial growth were obtained after 13 days of cultivation at media volume of 100 ml, 100 rpm, initial pH 4.5, and 25degrees C. The best mycelial growth was observed when MgSO4 . 7H2O and D-sucrose were added as a supplement in SFW. SWM must be a remarkable medium for L. edodes because of its simple preparation and low cost.
