Cloning and Phylogenetic Analysis of Chitin Synthase Genes from Tricholoma matsutake.
- Author:
Seok Jong SUH
1
;
Il Hyeon KIM
;
Ju Hyun NAM
;
Sa Youl GHIM
;
Kyung Sook BAE
;
Jong Guk KIM
Author Information
1. Department of Microbiology, College of Natural Sciences, Kyungpook National University, 1370 Sangyuk-dong, Daegu 702-701, Korea. kimjg@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Tricholoma matsutake;
Chitin synthase
- MeSH:
Agaricales;
Base Sequence;
Chitin Synthase*;
Chitin*;
Clone Cells*;
Cloning, Organism*;
Transferases;
Tricholoma*
- From:Mycobiology
2001;29(4):179-182
- CountryRepublic of Korea
- Language:English
-
Abstract:
Chitin synthases(UDP-N-acetyl-D-glucosamine: chitin 4-beta-N-acetyl-D-glucosaminyl transferase, EC 2.4.1.16) catalyze the synthesis of chitin from UDP-N-acetyl-D-glucosamine. Two zymogenic type of chitin synthase gene(TmCHS1 and TmCHS2) were amplified and its nucleotide sequences were determined. By the amino acid comparison and UPGMA tree grouping, TmChs1 and TmChs2 were classified as class II and class IV chitin synthases respectively. The class II type TmChs1 was grouped with others of Agaricales ectomycorrhizal mushroom. Additionally the phylogenetic tree was well adapted to Hymenomycete previously classified by morphological and physiological characteristics.
