Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes.
- Author:
Jung Hee CHO
1
;
Seung Eun LEE
;
Who Bong CHANG
;
Jae Soon CHA
Author Information
1. Department of Agricultural Biology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea. jscha@cbnu.ac.kr
- Publication Type:Brief Communication
- Keywords:
Agrobacterium;
Flammulina velutipes;
Gene tagging;
Marker inheritance;
Transformation
- MeSH:
Agaricales*;
Agrobacterium;
Animals;
DNA;
Flammulina*;
Fruiting Bodies, Fungal;
Genome, Fungal;
Gills
- From:Mycobiology
2006;34(2):104-107
- CountryRepublic of Korea
- Language:English
-
Abstract:
Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. Transformation or gene tagging with Agrobacterium T-DNA based vector should be useful for wide ranges of genetic or molecular biological studies of the mushroom.
