Detection of Cellulolytic Activity in Ophiostoma and Leptographium species by Chromogenic Reaction.
- Author:
Min Woo HYUN
1
;
Ji Hwan YOON
;
Wook Ha PARK
;
Seong Hwan KIM
Author Information
1. Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan, Chungnam 330-714, Korea. piceae@naver.com
- Publication Type:Brief Communication
- Keywords:
Cellulolytic enzyme;
Chromogenic reaction;
Leptographium sp.;
Ophiostoma sp.;
Sapstaining fungi
- MeSH:
Cellulose;
Coloring Agents;
Culture Media;
Fungi;
Ophiostoma*;
Phenolsulfonphthalein
- From:Mycobiology
2006;34(2):108-110
- CountryRepublic of Korea
- Language:English
-
Abstract:
To understand the ability of producing cellulolytic enzyme activity in the sapstaining fungi, four species of Ophiostoma and two species of Leptographium were investigated in the culture media containing each of cellulose substrates such as CM-cellulose, Avicel and D-cellobiose and each of chromogenic dyes such as Congo-Red, Phenol Red, Remazol Brilliant Blue and Tryphan Blue. When the fungi were grown for 5~7 days at 25degrees C, the formation of clear zone by chromogenic reaction around the margin of the fungal colony was demonstrated in all the culture media Congo-Red containing CM-cellulose. There was difference in the formation of clear zone among the dyes. Only Ophiostoma setosum and Leptographium spp. showed cellulolytic activity to the three substrates. Overall, the results of this study show that ophiostomatoid sapstaining fungi can produce cellulolytic enzymes.
