Assessment of Antioxidant and Phenolic Compound Concentrations as well as Xanthine Oxidase and Tyrosinase Inhibitory Properties of Different Extracts of Pleurotus citrinopileatus Fruiting Bodies.
- Author:
Nuhu ALAM
1
;
Ki Nam YOON
;
Kyung Rim LEE
;
Hye Young KIM
;
Pyung Gyun SHIN
;
Jong Chun CHEONG
;
Young Bok YOO
;
Mi Ja SHIM
;
Min Woong LEE
;
Tae Soo LEE
Author Information
1. Division of Life Sciences, University of Incheon, Incheon 406-840, Korea. tslee@incheon.ac.kr
- Publication Type:Original Article
- Keywords:
Antioxidant;
Phenolic compounds;
Pleurotus citrinopileatus;
Tyrosinase inhibition;
Xanthine oxidase
- MeSH:
Acetone;
Acetonitriles;
Antioxidants;
Biphenyl Compounds;
Chlorogenic Acid;
Chromatography, Liquid;
Coumaric Acids;
Flavanones;
Fruit;
Gallic Acid;
Hesperidin;
Humans;
Hydrochloric Acid;
Hydroxybenzoates;
Isoflavones;
Methanol;
Monophenol Monooxygenase;
Phenol;
Picrates;
Pleurotus;
Reactive Oxygen Species;
Water;
Xanthine;
Xanthine Oxidase
- From:Mycobiology
2011;39(1):12-19
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cellular damage caused by reactive oxygen species has been implicated in several diseases, thus establishing a significant role for antioxidants in maintaining human health. Acetone, methanol, and hot water extracts of Pleurotus citrinopileatus were evaluated for their antioxidant activities against beta-carotene-linoleic acid and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing power, ferrous ion-chelating abilities, and xanthine oxidase inhibitory activities. In addition, the tyrosinase inhibitory effects and phenolic compound contents of the extracts were also analyzed. Methanol and acetone extracts of P. citrinopileatus showed stronger inhibition of beta-carotene-linoleic acid compared to the hot water extract. Methanol extract (8 mg/mL) showed a significantly high reducing power of 2.92 compared to the other extracts. The hot water extract was more effective than the acetone and methanole extracts for scavenging DPPH radicals. The strongest chelating effect (92.72%) was obtained with 1.0 mg/mL of acetone extract. High performance liquid chromatography analysis detected eight phenolic compounds, including gallic acid, protocatechuic acid, chlorogenic acid, ferulic acid, naringenin, hesperetin, formononetin, and biochanin-A, in an acetonitrile and hydrochloric acid (5 : 1) solvent extract. Xanthine oxidase and tyrosinase inhibitory activities of the acetone, methanol, and hot water extracts increased with increasing concentration. This study suggests that fruiting bodies of P. citrinopileatus can potentially be used as a readily accessible source of natural antioxidants.
