Enhanced Expression of Glucose 2-Oxidase in Phlebia tremellosa by Addition of Phthalates.
- Author:
Baikjoong KIM
1
;
Hyewon KIM
;
Hyoung T CHOI
Author Information
1. Department of Biochemistry, Kangwon National University, Chuncheon 200-701, Korea. htchoi@kangwon.ac.kr
- Publication Type:Brief Communication
- Keywords:
Hydrogen peroxide generation;
Phlebia tremellosa;
Pyranose oxidase
- MeSH:
Carbohydrate Dehydrogenases;
DNA, Complementary;
Fungi;
Glucose;
Glucose Oxidase;
Humans;
Hydrogen;
Hydrogen Peroxide;
Lignin;
Manganese;
Oxidoreductases;
Peroxidase;
Peroxidases;
Polymerase Chain Reaction;
Pyrrolidines
- From:Mycobiology
2011;39(1):64-66
- CountryRepublic of Korea
- Language:English
-
Abstract:
Most fungi possess several hydrogen peroxide-generating enzymes, glucose oxidase and pyranose oxidase. Pyranose oxidase can use glucose as its substrate to generate hydrogen peroxide. White rot fungi, which degrade diverse recalcitrant compounds, contain lignin-degrading enzymes, and lignin peroxidase and manganese peroxidase require hydrogen peroxide for their enzymatic reactions. In this study, we isolated a cDNA fragment of pyranose oxidase from Phlebia tremellosa using PCR and examined its expression under the degradation conditions of diethylphthalate (DEP). Pyranose oxidase expression was enhanced up to 30% by the addition of DEP, and this result supports the possible involvement of pyranose oxidase in the degradation of recalcitrant compounds.
