Multi-loci Molecular Characterisation of Endophytic Fungi Isolated from Five Medicinal Plants of Meghalaya, India.
- Author:
Ranjan Kumar BHAGOBATY
1
;
S R JOSHI
Author Information
1. Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Umshing, Shillong 793 022, Meghalaya, India. srjoshi2006@yahoo.co.in
- Publication Type:Original Article
- Keywords:
Cryptic morphology;
Endophytic fungi;
Identification;
Medicinal plants;
Molecular phylogeny
- MeSH:
Biodiversity;
Camellia;
Databases, Nucleic Acid;
DNA;
Fungi;
India;
Plants, Medicinal;
Potentilla;
Tubulin
- From:Mycobiology
2011;39(2):71-78
- CountryRepublic of Korea
- Language:English
-
Abstract:
The phylogenetic relationships of the most dominant and morphologically cryptic endophytic fungal isolates from each of five selected medicinal plants, namely Potentilla fulgens, Osbeckia stellata, Osbeckia chinensis, Camellia caduca, and Schima khasiana of the biodiversity rich state of Meghalaya, were assessed with random amplification of polymorphic DNA and PCR-restriction fragment length polymorphism profiles. Sequencing of the internal transcribed spacer 1, small subunit rRNA and partial beta-tubulin gene fragments was also conducted to determine the phylogenetic relationships of these isolates with fungal sequences available in Genbank, NCBI. The identity of the fungal isolates is suggested based on the molecular phylogenetic data.
