The Distribution of Cytoplasm and Nuclei within the Extra-radical Mycelia in Glomus intraradices, a Species of Arbuscular Mycorrhizal Fungi.
- Author:
Jaikoo LEE
1
Author Information
1. Gyeonggi Science High School for The Gifted, Suwon 440-800, Korea. jh0221hy@empal.com
- Publication Type:In Vitro ; Original Article
- Keywords:
Arbuscular mycorrhizae fungus spore;
Cytoplasm;
Glomus;
Nuclei;
Septum
- MeSH:
Collodion;
Cytoplasm;
DNA;
Fungal Structures;
Fungi;
Hyphae;
Indoles;
Membranes;
Mycelium;
Mycorrhizae;
Organic Chemicals;
Spores
- From:Mycobiology
2011;39(2):79-84
- CountryRepublic of Korea
- Language:English
-
Abstract:
Nuclear distribution within the extra-radical fungal structures and during spore production in the arbuscular mycorrhizae fungus Glomus intraradices was examined using an in vitro monoxenic culture system. A di-compartmental monoxenic culture system was modified using a nitrocellulose membrane and a coverglass slip for detailed observations. Nuclear distribution was observed using the fluorescent DNA binding probes SYBR Green I and DAPI. Both septate and non-septate mycelial regions were observed, but cytoplasmic contents were only found within non-septate mycelia. Nuclear fluorescent staining revealed that the non-septate hyphal region contained nuclei only with cytoplasm, and that nuclear distribution was limited by septa. Swollen hyphal bodies were often associated with septate and empty-looking hyphae. Cytoplasmic contents filled the swollen hyphal body from the non-septate hyphal region following removal of the septa. As a consequence, the swollen body developed into a new spore. These observations provide understanding about the distribution of AM fungal nuclei within extra-radical mycelia and during spore formation. The results suggest a mechanism by which the development of a cytoplasm-containing mycelium is controlled by the formation or removal of septa to efficiently maintain and proliferate essential contents. This mechanism may provide a survival strategy to the fungus.
