Growth and Cultural Characteristics of Ophiocordyceps longissima Collected in Korea.
- Author:
Gi Ho SUNG
1
;
Bhushan SHRESTHA
;
Sang Kuk HAN
;
Jae Mo SUNG
Author Information
1. Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 441-707, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Carbon source;
Growth characteristics;
Mineral salt;
Nitrogen source;
Ophiocordyceps longissima
- MeSH:
Agar;
Carbon;
Cultural Characteristics;
Fruit;
Glucose;
Hydrogen-Ion Concentration;
Korea;
Light;
Maltose;
Nitrogen;
Peptones;
Salts;
Schizophyllum;
Solanum tuberosum;
Yeasts
- From:Mycobiology
2011;39(2):85-91
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the effect of nutritional and environmental factors on Ophiocordyceps longissima mycelial growth. The longest colony diameter was observed on Schizophyllum (mushroom) genetics complete medium plus yeast extract, Schizophyllum (mushroom) genetics minimal medium, and Sabouraud dextrose agar (SDA); however, malt-extract yeast-extract agar, SDA plus yeast extract, yeast-extract malt-extract peptone dextrose agar, SDA, oatmeal agar, and potato dextrose agar showed higher mycelia density. A temperature of 25degrees C was optimum and 7.0 was the optimum pH for mycelial growth. Colony diameter was similar under light and dark conditions. Maltose and yeast extract showed the highest mycelial growth among carbon and nitrogen sources respectively. The effect of mineral salts was less obvious; however, K3PO4 showed slightly better growth than that of the other mineral salts tested. Among all nutrition sources tested, complex organic nitrogen sources such as yeast extract, peptone, and tryptone were best for mycelial growth of O. longissima. Ophiocordyceps longissima composite medium, formulated by adding maltose (2% w/v), yeast extract (1% w/v), and K3PO4 (0.05% w/v) resulted in slightly longer colony diameter. In vitro mycelial O. longissima growth was sustainable and the production of fruiting bodies could be used for commercial purposes in the future.
