Genetic Diversity and Pathogenicity of Cylindrocarpon destructans Isolates Obtained from Korean Panax ginseng.
- Author:
Jeong Young SONG
1
;
Mun Won SEO
;
Sun Ick KIM
;
Myeong Hyeon NAM
;
Hyoun Sub LIM
;
Hong Gi KIM
Author Information
1. Department of Applied Biology, Chungnam National University, Daejeon 305-764, Korea. hgkim@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Cylindrocarpon destructans;
Genetic diversity;
Ginseng root rot;
Panax ginseng;
Pathogenicity
- MeSH:
Base Sequence;
DNA, Ribosomal;
Genetic Variation*;
Panax*;
Sequence Analysis;
Virulence*
- From:Mycobiology
2014;42(2):174-180
- CountryRepublic of Korea
- Language:English
-
Abstract:
We analyzed the genetic diversity of Cylindrocarpon destructans isolates obtained from Korean ginseng (i.e., Panax ginseng) roots by performing virulence tests and nuclear ribosomal gene internal transcribed spacer (ITS) and mitochondrial small subunit (mt SSU) rDNA sequence analysis. The phylogenetic relationship analysis performed using ITS DNA sequences and isolates from other hosts helped confirm that all the Korean C. destructans isolates belonged to Nectria/Neonectria radicicola complex. The results of in vivo and ex vivo virulence tests showed that the C. destructans isolates could be divided into two groups according to their distinctive difference in virulence and the genetic diversity. The highly virulent Korean isolates in pathogenicity group II (PG II), together with foreign isolates from P. ginseng and P. quinquefolius, formed a single group. The weakly virulent isolates in pathogenicity group I, together with the foreign isolates from other host plants, formed another group and exhibited a greater genetic diversity than the isolates of PG II, as confirmed by the mt SSU rDNA sequence analysis. In addition, as the weakly virulent Korean isolates were genetically very similar to the foreign isolates from other hosts, they were likely to originate from hosts other than the ginseng plants.
