Neuraminidase Inhibitors from the Fermentation Broth of Phellinus linteus.
- Author:
Byung Soon HWANG
1
;
Myeong Seok LEE
;
Seung Woong LEE
;
In Kyoung LEE
;
Geon Sik SEO
;
Hwa Jung CHOI
;
Bong Sik YUN
Author Information
1. Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea. bsyun@jbnu.ac.kr
- Publication Type:Brief Communication
- Keywords:
4-(3,4-Dihydroxyphenyl)-3-buten-2-one;
Anti-influenza agent;
Inotilone;
Neuraminidase inhibitor;
Phellinus linteus
- MeSH:
Cytopathogenic Effect, Viral;
Fermentation*;
Fungi;
Inhibitory Concentration 50;
Madin Darby Canine Kidney Cells;
Neuraminidase*
- From:Mycobiology
2014;42(2):189-192
- CountryRepublic of Korea
- Language:English
-
Abstract:
During a search for neuraminidase inhibitors derived from medicinal fungi, we found that the fermentation broth of Phellinus linteus exhibited potent neuraminidase inhibitory activity. Through bioassay-guided fractionation, two active compounds were purified from the ethyl acetate-soluble portion of the fermentation broth of P. linteus. These structures were identified as inotilone (1) and 4-(3,4-dihydroxyphenyl)-3-buten-2-one (2) by spectroscopic methods. Compounds 1 and 2 inhibited H1N1 neuraminidase activity with IC50 values of 29.1 and 125.6 microM, respectively, in a dose-dependent manner. They also exhibited an antiviral effect in a viral cytopathic effect reduction assay using MDCK cells. These results suggest that compounds 1 and 2 from the culture broth of P. linteus would be good candidates for the prevention and therapeutic strategies towards viral infections.
