Antimicrobial and Cytotoxic Activity of Di-(2-ethylhexyl) Phthalate and Anhydrosophoradiol-3-acetate Isolated from Calotropis gigantea (Linn.) Flower.
10.4489/MYCO.2009.37.1.031
- Author:
M Rowshanul HABIB
1
;
M Rezaul KARIM
Author Information
1. Department of Biochemistry and Molecular Biology, Rajshahi University, Rajshahi-6205, Bangladesh. rezaplazad@yahoo.com
- Publication Type:Original Article
- Keywords:
Anhydrosophoradiol-3-acetate;
Antimicrobial;
Calotropis gigantea and Di-(2-ethylhexyl) phthalate
- MeSH:
Acetates;
Artemia;
Calotropis;
Chromatography;
Chromatography, Thin Layer;
Diffusion;
Flowers;
Microbial Sensitivity Tests;
Phthalic Acids;
Salts;
Silica Gel
- From:Mycobiology
2009;37(1):31-36
- CountryRepublic of Korea
- Language:English
-
Abstract:
A phytochemical study on the flowe r of Calotropis gigantea (Linn.) using silica gel column chromatography and preparative thin layer chromatography, led to the first time isolation of Di-(2-ethylhexyl) phthalate (compound 1) and anhydrosophoradiol-3-acetate (compound 2). The structures of these compounds were confirmed by spectroscopic analyses (IR, HRTOFMS and NMR). The antibacterial and antifungal activities of ethyl acetate extract, compound 1 and compound 2 were measured using the disc diffusion method. Ethyl acetate extract and compound 1 presented better results than compound 2. The minimum inhibitory concentrations (MICs) of the extract and compounds were found to be in the range of 16~128 microg/ml. The cytotoxicity (LC50) against brine shrimp nauplii (Artemia salina) were also evaluated and found to be 14.61 microg/ml for ethyl acetate, 9.19 microg/ml for compound 1 and 15.55 microg/ml for compound 2.
