Probable Role of Beta 2-Adrenergic Receptor Gene Haplotype in Toluene Diisocyanate-Induced Asthma.
10.4168/aair.2010.2.4.260
- Author:
Young Min YE
1
;
Young Mi KANG
;
Seung Hyun KIM
;
Hyun Young LEE
;
Cheol Woo KIM
;
Choon Sik PARK
;
Chein Soo HONG
;
Hae Sim PARK
Author Information
1. Department of Allergy & Rheumatology, Ajou University School of Medicine, Suwon, Korea. hspark@ajou.ac.kr
- Publication Type:Original Article
- Keywords:
Isocyanate;
asthma;
beta2-adrenergic receptor;
specific IgE;
genetic susceptibility
- MeSH:
Antibodies;
Asthma;
Enzyme-Linked Immunosorbent Assay;
Genetic Predisposition to Disease;
Haplotypes;
Homozygote;
Humans;
Immunoglobulin E;
Immunoglobulin G;
Mass Screening;
Phenotype;
Polymorphism, Genetic;
Polymorphism, Single Nucleotide;
Serum Albumin;
Toluene;
Toluene 2,4-Diisocyanate
- From:Allergy, Asthma & Immunology Research
2010;2(4):260-266
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: A genetic polymorphism of the beta 2-adrenergic receptor is a major factor associated with the asthmatic phenotype. The association of this polymorphism with toluene diisocyanate (TDI)-induced asthma has not been investigated. We examined 103 TDI-induced asthma patients (TDI-OA), 60 asymptomatic exposed controls (AEC), and 263 unexposed healthy controls (NC) in order to identify beta 2-adrenergic receptor gene (ADRB2) polymorphisms and the possible association with TDI-induced asthma. METHODS: Single nucleotide polymorphisms (SNPs) of ADRB2 were genotyped by direct sequencing. Serum-specific IgE and IgG levels were measured using an enzyme-linked immunosorbent assay. Phenotypes and clinical patient parameters were compared. RESULTS: SNPs were identified (-47 T>C, -20 T>C, Arg16Gly A>G, Gln27Glu C>G, Leu134Leu G>A, Arg175Arg C>A) during ADRB2 screening (from -231 to 793 bp). No significant differences in allelic and genotypic frequencies were noted for any of the six ADRB2 SNPs. The Arg16Gly A>G, Leu134Leu G>A, and Arg175Arg C>A SNPs and haplotype 1 [TTACGC] were significantly associated with specific IgE antibodies to the TDI-human serum albumin (HSA) conjugate in TDI-exposed subjects (P<0.05). Exposed workers with the ADRB2 ht1/ht1 homozygote had a significantly higher TDI-HSA conjugate-specific IgE sensitization rate than did those with the null ht1 haplotype (odds ratio, 15.40; 95% confidence interval, 1.81-131.06). CONCLUSIONS: ADRB2 polymorphisms may affect IgE-specific sensitization to TDI-HSA conjugate in TDI-exposed workers.
