Effect of kainic acid on the phosphorylation of mitogen activated protein kinases in rat hippocampus.
- Author:
Je Seong WON
1
;
Jin Koo LEE
;
Seong Soo CHOI
;
Dong Keun SONG
;
Sung Oh HUH
;
Yung Hi KIM
;
Hong Won SUH
Author Information
1. Department of Pharmacology, College of Medicine, Institute of Natural Medicine, Hallym University, 1 Okcheon-dong, Chuncheon, 200-702, Korea. hwsuh@hallym.ac.kr
- Publication Type:Original Article
- MeSH:
Animals;
Cycloheximide;
Hippocampus*;
Kainic Acid*;
Mitogen-Activated Protein Kinase Kinases;
Mitogen-Activated Protein Kinases*;
Phosphorylation*;
Phosphotransferases;
Rats*
- From:The Korean Journal of Physiology and Pharmacology
2001;5(6):451-456
- CountryRepublic of Korea
- Language:English
-
Abstract:
In rat hippocampus, kainic acid (KA; 10 mg/kg; i.p.) increased the phosphorylated forms of ERK1/2 (p-ERK1/2) and Jun kinase1 (p-JNK1), but not p-JNK2 and p38 (p-p38). The preadministration with cycloheximide (CHX; 5 mg/kg; i.p.) inhibited KA-induced increase of p-JNK1, but not p-ERK1/2. Surprisingly, the phosphorylated upstream MAP kinase kinases (p-MKKs) were not correlated with their downstream MAP kinases. The basal p-MKK1/2 levels were completely abolished by KA, which were reversed by CHX. In addition, p-MKK4 and p-MKK3/6 levels were enhanced by CHX alone, but were attenuated by KA. Thus, our results showed that KA increased the p-ERK and p-JNK levels in rat hippocampus, which were not parallel with their classical upstreamal kinases.
