Effects of gamma-Aminobutyric Acid on Pancreatic Amylase Secretion Evoked by Sodium Oleate in Anesthetized Rats.
- Author:
Yong Deuk PARK
1
;
Zheng Yun CUI
;
Hyung Seo PARK
;
Hyoung Jin PARK
Author Information
1. Department of Physiology, College of Medicine, The Hallym Academy of Sciences, Hallym University, Chuncheon, Korea. hjpark@hallym.ac.kr
- Publication Type:Original Article
- Keywords:
GABA;
Sodium oleate;
Cholecystokinin;
Secretin;
Pancreatic exocrine secretion;
GABAA- receptor
- MeSH:
Amylases*;
Animals;
Bicuculline;
Cholecystokinin;
gamma-Aminobutyric Acid*;
Islets of Langerhans;
Oleic Acid*;
Pancreas;
Rats*;
Secretin;
Sodium*;
Urethane;
Water
- From:The Korean Journal of Physiology and Pharmacology
2002;6(1):27-32
- CountryRepublic of Korea
- Language:English
-
Abstract:
gamma-Aminobutyric Acid (GABA) is contained in pancreatic islet beta-cells although its physiological role in pancreatic exocrine function is completely unknown at the present time. Recently, we have reported that exogenous GABA enhances secretagogue-evoked exocrine secretion in the isolated, perfused rat pancreas. This study was aimed to investigate an effect of exogenous GABA on pancreatic exocrine secretion in vivo evoked by intestinal stimulation. Rats were anesthetized with urethane (1.4 g/kg) after 24-h fast with free access to water. GABA (10, 30 and 100micromol/kg/h), given intravenously, did not change spontaneous pancreatic amylase secretion but dose-dependently elevated the amylase secretion evoked by intraduodenal sodium oleate (0.05 mmol/h). GABA (30micromol/kg/h) also further increased the amylase secretion stimulated by CCK+(30 pmol/kg/h) plus secretin (20 pmol/kg/h) but failed to modify the amylase secretion induced by secretin alone. GABA (10, 30 and 100micromol/kg/h) also dose-dependently elevated pancreatic amylase secretion evoked by CCK+alone. Bicuculline (100micromol/kg/h), a GABAA-receptor antagonist, markedly reduced the GABA-enhanced pancreatic responses to sodium oleate, CCK+plus secretin or CCK+alone. The results indicate that GABA enhances the sodium oleate-evoked pancreatic amylase secretion via GABAA-receptors in anesthetized rats, which may account for elevating the action of CCK+released by sodium oleate.
