Resveratrol raises in vitro anticancer effects of paclitaxel in NSCLC cell line A549 through COX-2 expression.
10.4196/kjpp.2017.21.5.465
- Author:
Fanhua KONG
1
;
Runqi ZHANG
;
Xudong ZHAO
;
Guanlin ZHENG
;
Zhou WANG
;
Peng WANG
Author Information
1. School of Medicine, Shandong University, Jinan 250000, Shandong, China.
- Publication Type:Original Article
- Keywords:
A549;
Cancer;
Gene;
Paclitaxel;
Resveratrol
- MeSH:
Carcinoma, Non-Small-Cell Lung;
Caspase 3;
Caspase 8;
Caspase 9;
Cell Line*;
Collagen;
Fibroblasts;
Flow Cytometry;
Humans;
In Vitro Techniques*;
Lung;
Paclitaxel*;
Procollagen;
RNA, Messenger;
Tissue Inhibitor of Metalloproteinase-1;
Tissue Inhibitor of Metalloproteinase-2;
Tissue Inhibitor of Metalloproteinase-3;
Vascular Endothelial Growth Factor A
- From:The Korean Journal of Physiology and Pharmacology
2017;21(5):465-474
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to determine the raising anticancer effects of resveratrol (Res) on paclitaxel (PA) in non-small cell lung cancer (NSCLC) cell line A549. The 10 µg/ml of Res had no effect on human fetal lung fibroblast MRC-5 cells or on A549 cancer cells and the 5 or 10 µg/ml of PA also had no effect on MRC-5 normal cells. PA-L (5 µg/ml) and PA-H (10 µg/ml) had the growth inhibitory effects in NSCLC cell line A549, and Res increased these growth inhibitory effects. By flow cytometry experiment, after Res (5 µg/ml)+PA-H (10 µg/ml) treatment, the A549 cells showed the most apoptosic cells compared to other group treatments, and after additional treatment with Res, the apoptosic cells of both two PA concentrations were raised. Res+PA could reduce the mRNA and protein expressions of COX-2, and Res+PA could reduce the COX-2 related genes of VEGF, MMP-1, MMP-2, MMP-9, NF-κB, Bcl-2, Bcl-xL, procollagen I, collagen I, collagen III and CTGF, TNF-α, IL-1β, iNOS and raise the TIMP-1, TIMP-2, TIMP-3, IκB-α, p53, p21, caspase-3, caspase-8, caspase-9, Bax genes compared to the control cells and the PA treated cells. From these results, it can be suggested that Res could raise the anticancer effects of PA in A549 cells, thus Res might be used as a good sensitizing agent for PA.
