Water Extract of Samultang Reduces Apoptotic Cell Death by H2O2-Induced Oxidative Injury in SK-N-MC Cells.
10.4196/kjpp.2009.13.3.139
- Author:
Gyoung Wan LEE
1
;
Min Sun KIM
Author Information
1. Department of Physiology, Wonkwang University School of Medicine, Iksan 570-749, Korea. mskim@wku.ac.kr
- Publication Type:Original Article
- Keywords:
Samultang;
Apoptosis;
Oxidative injury;
SK-N-MC
- MeSH:
Apoptosis;
Asian Continental Ancestry Group;
Caspase 3;
Cell Death;
DNA;
Drugs, Chinese Herbal;
Electrophoresis;
Humans;
Membrane Potentials;
Optical Imaging;
Oxidative Stress;
Sepharose;
Water
- From:The Korean Journal of Physiology and Pharmacology
2009;13(3):139-145
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study was to evaluate the effects of the water extract of Samultang (SMT), a Chinese herb, on apoptotic cell death by H2O2-induced oxidative stress in SK-N-MC cells. A nuclear fragmentation was observed via fluorescence imaging 12 h after exposure to 30 micrometer H2O2 and DNA laddering was detected via agarose electrophoresis gel. In addition, increases in sub-G1 phase and cleavage of the PARP protein were observed. However, treatment with SMT for 2 h prior to H2O2 exposure significantly reduced apoptotic cell death induced by incubation with 30 micrometer H2O2 in SK-N-MC cells. Pre-incubation with water extract of SMT for 2 h prevented the H2O2-induced decrease in mitochondrial transmembrane potential. SMT also attenuated the increase in caspase-3 activity and the breakdown of PARP protein caused by H2O2-induced oxidative stress. These results suggest that the water extract of SMT provides inhibition of apoptotic cell death against oxidative injury in SK-N-MC cells.
