Ischemia/reperfusion Lung Injury Increases Serum Ferritin and Heme Oxygenase-1 in Rats.
10.4196/kjpp.2009.13.3.181
- Author:
Yoon Yub PARK
1
Author Information
1. Department of Physiology, School of Medicine, Catholic University of Daegu, Daegu 705-718, Korea. yypark@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Ferritin;
Heme oxygenase-1;
Ischemia/reperfusion;
Acute lung injury;
ARDS
- MeSH:
Acute Lung Injury;
Animals;
Bronchoalveolar Lavage;
Ferritins;
Heme;
Heme Oxygenase-1;
Humans;
Imidazoles;
Leukocyte Count;
Lung;
Lung Injury;
Nitro Compounds;
Oxidative Stress;
Quinacrine;
Rats;
Respiratory Distress Syndrome, Adult;
Salicylamides
- From:The Korean Journal of Physiology and Pharmacology
2009;13(3):181-187
- CountryRepublic of Korea
- Language:English
-
Abstract:
Intestinal ischemia/reperfusion (I/R) is one of common causes of acute lung injury (ALI). Early and accurate diagnosis of patients who are like to develop serious acute respiratory distress syndrome (ARDS) would give a therapeutic advantage. Ferritin and heme oxygenase-1 (HO-1) are increased by oxidative stress and are potential candidates as a predictive biomarker of ARDS. However, the mechanisms responsible for the increases of ferritin and HO-1, and their relationship to ALI, are unclear. In order to elucidate the interactions between ferritin and HO-1, we studied the changes in ferritin and HO-1 levels in serum and bronchoalveolar lavage (BAL) fluid after intestinal I/R injury in rats. Leukocyte number and protein contents in BAL fluid were elevated following I/R, and the increases were attenuated by mepacrine pretreatment. Both serum ferritin and HO-1 concentrations were progressively elevated throughout the 3 h observation period. Mepacrine pretreatment attenuated the increase of serum and BAL fluid ferritin concentrations, but did not suppress the increase of serum HO-1. Moreover, BAL fluid HO-1 levels did not change after I/R or after mepacrine pretreated I/R compared with sham rats. Unlike ferritin, HO-1 levels are not exactly matched with the ALI. Therefore, there might be a different mechanism between the changes of ferritin and HO-1 in intestinal I/R-induced ALI model.
