Effects of exogenous ATP on calcium mobilization and cell proliferation in C6 glioma cell.
- Author:
Eun Jung LEE
1
;
Seok Ho CHA
;
Won Kyu LEE
;
Kweon Hakeng LEE
;
Sang Bok LEE
Author Information
1. Department of Pharmacology, Catholic University Medical College, 505 Banpo-dong, Seocho-gu, Seoul 137-701 South Korea.
- Publication Type:Original Article
- Keywords:
ATP, Purinoceptor;
C6 glioma cell;
Cytosolic free calcium;
Cell proliferation
- MeSH:
Adenosine;
Adenosine Triphosphate*;
Calcium*;
Cell Proliferation*;
Cytosol;
Glioma*;
Ionomycin;
Receptors, Purinergic;
Suramin;
Type C Phospholipases
- From:The Korean Journal of Physiology and Pharmacology
1998;2(4):419-425
- CountryRepublic of Korea
- Language:English
-
Abstract:
To clarify the effect of extracellular ATP in cultured C6 glioma cells, ATP-induced cytosolic free calcium ((Ca2+)i) mobilization and cell proliferation were investigated. ATP-induced (Ca2+)i increased in a dose-dependent manner (10-7 M apprx 10-3 M). ATP-induced (Ca2+)i increases were slightly slowed in extracellular calcium-free conditions especially in sustained phase. ATP-induced (Ca2+)i increment was also inhibited by the pretreatment of U73122, a phospholipase C (PLC) inhibitor, in a time-dependent manner. Suramin, a putative P2Y receptor antagonist, dose-dependently weakened ATP-induced (Ca2+)i mobilization. Significant increases in cell proliferation were observed at 2, 3, and 4 days after ATP was added. Stimulated cell proliferation was also observed with adenosine at days 2 and 3. This cell proliferation was significantly inhibited by the treatment with suramin. Ionomycin also stimulated cell proliferation in a concentration-dependent manner. In conclusion, we suggest that extracellular ATP stimulates C6 glioma cell proliferation via intracellular free calcium mobilization mediated by purinoceptor.
