Effect of the inhibition of platelet activating factor on oxidative lung injury induced by interleukin-1 alpha.
- Author:
Young Man LEE
1
;
Yoon Yub PARK
Author Information
1. Department of Physiology, School of Medicine, Catholic University of Taegu-Hyosung, Taegu 705-034, South Korea.
- Publication Type:Original Article
- Keywords:
Acute lung leak;
Interleukin-1;
PAF, NFkB;
Oxidative stress
- MeSH:
Acute Lung Injury;
Animals;
Blood Platelets*;
Bronchoalveolar Lavage Fluid;
Cytokines;
Endothelial Cells;
Hydrogen Peroxide;
Injections, Intravenous;
Insufflation;
Interleukin-1*;
Interleukin-1alpha*;
Lung Injury*;
Lung*;
Microscopy, Electron;
Neutrophils;
NF-kappa B;
Oxidative Stress;
Peroxidase;
Phospholipases A2;
Platelet Activating Factor*;
Rats;
Respiratory Burst;
Respiratory Distress Syndrome, Adult;
Trachea
- From:The Korean Journal of Physiology and Pharmacology
1998;2(4):479-491
- CountryRepublic of Korea
- Language:English
-
Abstract:
In order to know the pathogenesis of adult respiratory distress syndrome (ARDS) in association with the oxidative stress by neutrophils, the role of platelet activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, PAF) was investigated during acute lung injury induced by interleukin-1alpha (IL-1) in rats. An insufflation of IL-1 into the rat's trachea increased the acetyltransferase activity in the lung and the increase of PAF content was followed. As evidences of acute lung injury by neutrophilic respiratory burst, lung leak index, myeloperoxidase activity, numbers of neutrophils in the bronchoalveolar lavage fluid, neutrophilic adhesions to endothelial cells and NBT positive neutrophils were increased after IL-1 treatment. In addition, a direct instillation of PAF into the trachea caused acute lung leak and the experimental results showed a similar pattern in comparison with IL-1 induced acute lung injury. For the confirmation of oxidative stress during acute lung leak by IL-1 and PAF, a histochemical electron microscopy was performed. In IL-1 and PAF treated lungs of rats, the deposits of cerrous perhydroxide were found. To elucidate the role of PAF, an intravenous injection of PAF receptor antagonist, WEB 2086 was given immediately after IL-1 or PAF treatment. WEB 2086 decreased the production of hydrogen peroxide and the acute lung leak. In ultrastructural study, WEB 2086 mitigated the pathological changes induced by IL-1 or PAF. The nuclear factor kappa B (NFkB) was activated by PAF and this activation was inhibited by WEB 2086 almost completely. Based on these experimental results, it is suggested that the PAF produced in response to IL-1 through the remodeling pathway has the major role for acute lung injury by neutrophilic respiratory burst. In an additional experiment, we can also come to conclude that the activation of the NFkB by PAF is thought to be the fundamental mechanism to initiate the oxidative stress by neutrophils causing release of proinflammatory cytokines and activation of phospholipase A2.
