Diversity of Ion Channels in Human Bone Marrow Mesenchymal Stem Cells from Amyotrophic Lateral Sclerosis Patients.
10.4196/kjpp.2008.12.6.337
- Author:
Kyoung Sun PARK
1
;
Mi Ran CHOI
;
Kyoung Hwa JUNG
;
Seung Hyun KIM
;
Hyun Young KIM
;
Kyung Suk KIM
;
Eun Jong CHA
;
Yangmi KIM
;
Young Gyu CHAI
Author Information
1. Division of Molecular and Life Sciences, Hanyang University, Ansan, 426-791, Korea.
- Publication Type:Original Article
- Keywords:
Bone marrow;
Stem cells;
Functional ion channels;
Tetrodotoxin-sensitive Na+ current;
Passage-dependency
- MeSH:
Amyotrophic Lateral Sclerosis;
Bone Marrow;
Humans;
Ion Channels;
Mesenchymal Stromal Cells;
Stem Cells
- From:The Korean Journal of Physiology and Pharmacology
2008;12(6):337-342
- CountryRepublic of Korea
- Language:English
-
Abstract:
Human bone marrow mesenchymal stem cells (hBM-MSCs) represent a potentially valuable cell type for clinical therapeutic applications. The present study was designed to evaluate the effect of long-term culturing (up to 10th passages) of hBM-MSCs from eight individual amyotrophic lateral sclerosis (ALS) patients, focusing on functional ion channels. All hBM-MSCs contain several MSCs markers with no significant differences, whereas the distribution of functional ion channels was shown to be different between cells. Four types of K+ currents, including noise-like Ca+2-activated K+ current (IKCa), a transient outward K+ current (Ito), a delayed rectifier K+ current (IKDR), and an inward-rectifier K+ current (Kir) were heterogeneously present in these cells, and a TTX-sensitive Na+ current (INa,TTX) was also recorded. In the RT-PCR analysis, Kv1.1, heag1, Kv4.2, Kir2.1, MaxiK, and hNE-Na were detected. In particular, INa,TTX showed a significant passage-dependent increase. This is the first report showing that functional ion channel profiling depend on the cellular passage of hBM-MSCs
