Cloning of mouse AQP-CD gene.
- Author:
Jin Sup JUNG
1
;
Joo In KIM
;
Sae Ok OH
;
Mi Young PARK
;
Hae Rhan BAE
;
Sang Ho LEE
Author Information
1. Department of Physiology, College of Medicine, Pusan National University, 1 Ga 10, Ami-Dong, Suh-Gu, Pusan 602-739 South Korea.
- Publication Type:Original Article
- Keywords:
Aquaporin-CD;
Gene;
Cloning
- MeSH:
Animals;
Aquaporin 2;
Aquaporins;
Bacteriophages;
Clone Cells*;
Cloning, Organism*;
Consensus Sequence;
DNA;
Exons;
Genomic Library;
Humans;
Membranes;
Mice*;
Restriction Mapping;
TATA Box;
Tissue Distribution;
Vasopressins;
Water
- From:The Korean Journal of Physiology and Pharmacology
1997;1(2):195-200
- CountryRepublic of Korea
- Language:English
-
Abstract:
Water transport in highly-permeable membranes is facilitated by some specialized pathways, which are called aquaporins (AQP). AQP1 (AQP-CHIP) is the first recognized aquaporin identified from red cells and renal proximal tubules. Up until now 4 other aquaporin homologs have been reported. Each aquaporin has its unique tissue distribution and regulatory mechanisms. To elucidate molecular mechanisms for their transcription regulation and tissue-specific expression isolation of aquaporin genes is required. To clone promoters of the AQP family mouse genomic library was screened by the 1st exon-specific probe of AQP4, and 5 different plaques were positively hybridized. Phage DNAs were purified and characterized by restriction mapping and sequencing. One of them is the mouse AQP-CD gene. The gene was consisted of 4 exons and the exon-intron boundaries of mouse AQP-CD gene were identified at identical positions in other related genes. The 5'-flanking region of AQP-CD gene contains one classic TATA box, a GATA consensus sequence, an E-box and a cyclic AMP-responsive element. The cloning of the mouse AQP-CD gene, of which product is expressed in the collecting duct and is responsible for antidiuresis by vasopressin, will contribute to understand the molecular mechanisms of tissue-specific expression and regulation of AQP-CD gene under various conditions.
