Effects of Apigenin on Glutamate-induced Ca2+i Increases in Cultured Rat Hippocampal Neurons.
10.4196/kjpp.2008.12.2.43
- Author:
Ji Hwa HAN
1
;
Ki Jung KIM
;
Hyun Jong JANG
;
Ju Ho JANG
;
Myung Jun KIM
;
Ki Wug SUNG
;
Duck Joo RHIE
;
Yang Hyeok JO
;
Sang June HAHN
;
Mun Yong LEE
;
Shin Hee YOON
Author Information
1. Department of Physiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea. s-hyoon@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Apigenin;
Intracellular calcium;
Hippocampal neuron;
Glutamate;
Flavonoid
- MeSH:
Animals;
Apigenin;
Caffeine;
Calcium;
Calcium Signaling;
Glutamic Acid;
N-Methylaspartate;
Neurons;
Rats;
Receptors, Glutamate;
Receptors, Metabotropic Glutamate
- From:The Korean Journal of Physiology and Pharmacology
2008;12(2):43-49
- CountryRepublic of Korea
- Language:English
-
Abstract:
Flavonoids have been shown to affect calcium signaling in neurons. However, there are no reports on the effect of apigenin on glutamate-induced calcium signaling in neurons. We investigated whether apigenin affects glutamate-induced increase of free intracellular Ca2+concentration ([Ca2+]i) in cultured rat hippocampal neurons, using fura-2-based digital calcium imaging and microfluorimetry. The hippocampal neurons were used between 10 and 13 days in culture from embryonic day 18 rats. Pretreatment of the cells with apigenin (1micrometerto 100micrometer for 5 min inhibited glutamate (100 micrometer 1 min) induced [Ca2+]i increase, concentration-dependently. Pretreatment with apigenin (30micrometer for 5 min significantly decreased the [Ca2+]i responses induced by two ionotropic glutamate receptor agonists, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic (AMPA, 10 micrometer 1 min) and N-methyl-D-aspartate (NMDA, 100 micrometer 1 min), and significantly inhibited the AMPA-induced peak currents. Treatment with apigenin also significantly inhibited the [Ca2+]i response induced by 50 mM KCl solution, decreased the [Ca2+]i responses induced by the metabotropic glutamate receptor agonist, (S)-3,5-dihydroxyphenylglycine (DHPG, 100micrometer 90 s), and inhibited the caffeine (10 mM, 2 min)-induced [Ca2+]i responses. Furthermore, treatment with apigenin (30micrometer significantly inhibited the amplitude and frequency of 0.1 mM [Mg2+o-induced [Ca2+]i spikes. These data together suggest that apigenin inhibits glutamate-induced calcium signaling in cultured rat hippocampal neurons.
