Testosterone Relaxes Rabbit Seminal Vesicle by Calcium Channel Inhibition.
10.4196/kjpp.2008.12.2.73
- Author:
Jong Kok KIM
1
;
Woo Ha HAN
;
Moo Yeol LEE
;
Soon Chul MYUNG
;
Sae Chul KIM
;
Min Ky KIM
Author Information
1. Department of Physiology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea. heeyun@cau.ac.kr
- Publication Type:Original Article
- Keywords:
Testosterone;
Seminal vesicle;
Calcium channel;
Ejaculation
- MeSH:
4-Aminopyridine;
Calcium;
Calcium Channels;
Collagenases;
Contracts;
Ejaculation;
Glyburide;
Hand;
Ion Channels;
Male;
Muscle Cells;
Muscle, Smooth;
Norepinephrine;
Papain;
Peptide Hydrolases;
Peptides;
Relaxation;
Seminal Vesicles;
Testosterone;
Tetraethylammonium
- From:The Korean Journal of Physiology and Pharmacology
2008;12(2):73-77
- CountryRepublic of Korea
- Language:English
-
Abstract:
Recent studies have documented that testosterone relaxes several smooth muscles by modulating K+channel activities. Smooth muscles of seminal vesicles play a fundamental role in ejaculation, which might involve testosterone. This study was aimed to assess the role of testosterone in seminal vesicular motility by studying its effects on contractile agents and on the ion channels of single vesicular myocytes in a rabbit model. The contractile responses of circular smooth muscle strips of rabbit seminal vesicles to norepinephrine (10 micrometer), a high concentration of KCl (70 mM), and testosterone (10 micrometer were observed. Single vesicular myocytes of rabbit were isolated using proteolytic enzymes including collagenase and papain. Inside-out, attached, and whole-cell configurations were examined using the patch clamp technique. The applications of 10 micrometernorepinephrine or 70 mM KCl induced tonic contractions, and 10 micrometertestosterone (pharmacological concentration) evoked dose-dependent relaxations of these precontracted strips. Various K+channel blockers, such as tetraethylammonium (TEA; 10 mM), iberiotoxin (0.1 micrometer), 4-aminopyridine (4-AP, 10 micrometer, or glibenclamide (10 micrometer rarely affected these relaxations. Single channel data (of inside-out and attached configurations) of BK channel activity were also hardly affected by testosterone (10 micrometer). On the other hand, however, testosterone reduced L-type Ca2+currents significantly, and found to induce acute relaxation of seminal vesicular smooth muscle and this was mediated, at least in part, by Ca2+current inhibition in rabbit.
