(-)-Epigallocatechin Gallate Inhibits the Pacemaker Activity of Interstitial Cells of Cajal of Mouse Small Intestine.
10.4196/kjpp.2008.12.3.111
- Author:
Kweon Young KIM
1
;
Soo Jin CHOI
;
Hyuk Jin JANG
;
Dong Chuan ZUO
;
Pawan Kumar SHAHI
;
Shankar Prasad PARAJULI
;
Cheol Ho YEUM
;
Pyung Jin YOON
;
Seok CHOI
;
Jae Yeoul JUN
Author Information
1. Department of Rehabilitation, College of Medicine, Chosun University, Gwangju 501-759, Korea.
- Publication Type:Original Article
- Keywords:
(-)-epigallocatechin gallate (EGCG);
Interstitial cells of Cajal (ICC);
Pacemaker currents;
Intestinal motility
- MeSH:
Adenine;
Adenylyl Cyclases;
Animals;
Gastrointestinal Motility;
Glyburide;
Guanylate Cyclase;
Interstitial Cells of Cajal;
Intestine, Small;
Mice;
Patch-Clamp Techniques;
Tea
- From:The Korean Journal of Physiology and Pharmacology
2008;12(3):111-115
- CountryRepublic of Korea
- Language:English
-
Abstract:
The effects of (-)-epigallocatechin gallate (EGCG) on pacemaker activities of cultured interstitial cells of Cajal (ICC) from murine small intestine were investigated using whole-cell patch-clamp technique at 30degrees C and Ca2+ image analysis. ICC generated spontaneous pacemaker currents at a holding potential of -70 mV. The treatment of ICC with EGCG resulted in a dose-dependent decrease in the frequency and amplitude of pacemaker currents. SQ-22536, an adenylate cyclase inhibitor, and ODQ, a guanylate cyclase inhibitor, did not inhibit the effects of EGCG. EGCG-induced effects on pacemaker currents were not inhibited by glibenclamide, an ATP-sensitive K+ channel blocker and TEA, a Ca2+-activated K+ channel blocker. Also, we found that EGCG inhibited the spontaneous [Ca2+]i oscillations in cultured ICC. In conclusion, EGCG inhibited the pacemaker activity of ICC and reduced [Ca2+]i oscillations by cAMP-, cGMP-, ATP-sensitive K+channel-independent manner.
