Matrix Metallopeptidase 2 Gene Polymorphism is Associated with Obesity in Korean Population.
10.4196/kjpp.2008.12.3.125
- Author:
Dong Hee HAN
1
,
2
;
Su Kang KIM
;
Sungwook KANG
;
Bong Keun CHOE
;
Keon Sik KIM
;
Joo Ho CHUNG
Author Information
1. Kohwang Medical Research Institute, Kyung Hee University, Seoul 130-701, Korea. flyexcel@hanmail. net
2. jhchung@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Body mass index;
Linkage disequilibrium;
Matrix metallopeptidase 2;
Obesity;
Single nucleotide polymorphism
- MeSH:
Basement Membrane;
Body Mass Index;
Clinical Coding;
Haplotypes;
Lidocaine;
Linkage Disequilibrium;
Obesity;
Polymorphism, Single Nucleotide;
Proteins
- From:The Korean Journal of Physiology and Pharmacology
2008;12(3):125-129
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to determine whether single nucleotide polymorphisms (SNPs) of matrix metallopeptidase 2 (MMP2) are associated with obesity. MMP2 is an enzyme with proteolytic activity against matrix and nonmatrix proteins, particularly basement membrane constituents. To identify the relationship between polymorphisms of MMP2 and overweight/obese, we genotyped 5 SNPs (rs17242319, rs1053605, rs243849, rs2287074, and rs10775332) of the coding region of MMP2 using the Golden Gate assay on an Illumina BeadStation 500 GX. One hundred and forty two overweight/ obese (BMI > or =23) and 145 normal (BMI 18 to <23) subjects were analyzed. SNPStats, Haploview, HapAnalyzer, SNPAnalyzer, and Helixtree programs were used for the analysis of genetic data. A linkage disequilibrium (LD) block was discovered among the 5 SNPs selected, including rs17242319, rs1053605, rs243849, and rs2287074. Of the 5 polymorphisms, 2 synonymous SNPs [rs17242319 (Gly226Gly) and rs10775332 (Phe602Phe)] were found significant associations with overweight/obese. Recently, rs1132896 replaced rs17242319 as a new number (SNP database, BUILD 129). In haplotype analysis using Haploview, a haplotype (haplotype: CCCA) containing a meaningful polymorphism (rs17242319) was found to be significantly different. The results suggest that MMP2 may be associated with overweight/obese in Korean population.
