Paracrine influence of human perivascular cells on the proliferation of adenocarcinoma alveolar epithelial cells.
10.4196/kjpp.2017.21.2.161
- Author:
Eunbi KIM
1
;
Sunghun NA
;
Borim AN
;
Se Ran YANG
;
Woo Jin KIM
;
Kwon Soo HA
;
Eun Taek HAN
;
Won Sun PARK
;
Chang Min LEE
;
Ji Yoon LEE
;
Seung Joon LEE
;
Seok Ho HONG
Author Information
1. Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea. shhong@kangwon.ac.kr medfman@gmail.com
- Publication Type:Original Article
- Keywords:
Cancer;
Paracrine effect;
Perivascular cells;
Proliferation
- MeSH:
Adenocarcinoma*;
Carcinoma, Squamous Cell;
Coculture Techniques;
Culture Media, Conditioned;
Cytokines;
Epithelial Cells*;
Humans*;
In Vitro Techniques;
K562 Cells;
Leukemia, Erythroblastic, Acute;
Lung;
Lung Neoplasms;
Neoplasm Metastasis;
Umbilical Cord
- From:The Korean Journal of Physiology and Pharmacology
2017;21(2):161-168
- CountryRepublic of Korea
- Language:English
-
Abstract:
Understanding the crosstalk mechanisms between perivascular cells (PVCs) and cancer cells might be beneficial in preventing cancer development and metastasis. In this study, we investigated the paracrine influence of PVCs derived from human umbilical cords on the proliferation of lung adenocarcinoma epithelial cells (A549) and erythroleukemia cells (TF-1α and K562) in vitro using Transwell® co-culture systems. PVCs promoted the proliferation of A549 cells without inducing morphological changes, but had no effect on the proliferation of TF-1α and K562 cells. To identify the factors secreted from PVCs, conditioned media harvested from PVC cultures were analyzed by antibody arrays. We identified a set of cytokines, including persephin (PSPN), a neurotrophic factor, and a key regulator of oral squamous cell carcinoma progression. Supplementation with PSPN significantly increased the proliferation of A549 cells. These results suggested that PVCs produced a differential effect on the proliferation of cancer cells in a cell-type dependent manner. Further, secretome analyses of PVCs and the elucidation of the molecular mechanisms could facilitate the discovery of therapeutic target(s) for lung cancer.
