The oncogenic effects of p53-inducible gene 3 (PIG3) in colon cancer cells.
10.4196/kjpp.2017.21.2.267
- Author:
Seon Joo PARK
1
;
Hong Beum KIM
;
Jeeho KIM
;
Sanggon PARK
;
Seok Won KIM
;
Jung Hee LEE
Author Information
1. Laboratory of Genomic Instability and Cancer Therapeutics, Cancer Mutation Research Center, Chosun University School of Medicine, Gwangju 61452, Korea. jhlee75@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Cancer progression;
Colon cancer;
Oncogenesis;
PIG3
- MeSH:
Animals;
Carcinogenesis;
Cell Line;
Colon*;
Colonic Neoplasms*;
Fibroblasts;
Genes, vif;
HCT116 Cells;
Heterografts;
Humans;
In Vitro Techniques;
Mice;
Reactive Oxygen Species
- From:The Korean Journal of Physiology and Pharmacology
2017;21(2):267-273
- CountryRepublic of Korea
- Language:English
-
Abstract:
The p53-inducible gene 3 (PIG3), initially identified as a gene downstream of p53, plays an important role in the apoptotic process triggered by p53-mediated reactive oxygen species (ROS) production. Recently, several studies have suggested that PIG3 may play a role in various types of cancer. However, the functional significance of PIG3 in cancer remains unclear. Here, we found that PIG3 was highly expressed in human colon cancer cell lines compared to normal colonderived fibroblasts. Therefore, we attempted to elucidate the functional role of PIG3 in colon cancer. PIG3 overexpression increases the colony formation, migration and invasion ability of HCT116 colon cancer cells. Conversely, these tumorigenic abilities were significantly decreased in in vitro studies with PIG3 knockdown HCT116 cells. PIG3 knockdown also attenuated the growth of mouse xenograft tumors. These results demonstrate that PIG3 is associated with the tumorigenic potential of cancer cells, both in vitro and in vivo, and could play a key oncogenic role in colon cancer.
