Nitric Oxide Donor, NOR-3, Increased Expression of Cyclooxygenase-2, but not of Cyclooxygenase-1 in Cultured VSMC.
- Author:
Dong Hyup LEE
1
;
Ji Eun PARK
;
Young Jin KANG
;
Kwang Youn LEE
;
Hyoung Chul CHOI
Author Information
1. Department of Pharmacology, College of Medicine, Yeungnam University, Daegu, Korea. hcchoi@med.yu.ac.kr
- Publication Type:Original Article
- Keywords:
COX-2;
SNAP;
SNP;
NOR-3;
VSMC
- MeSH:
Animals;
Aorta, Thoracic;
Cyclooxygenase 1*;
Cyclooxygenase 2*;
Humans;
Inflammation;
Muscle, Smooth, Vascular;
Nitric Oxide*;
Rats;
Tissue Donors*
- From:The Korean Journal of Physiology and Pharmacology
2006;10(3):161-165
- CountryRepublic of Korea
- Language:English
-
Abstract:
NO and cyclooxygenase-2 (COX-2) are contributes to vascular inflammation induced by various stimulation. The mechanism, which explains a linkage between NO and COX-2, could be of importance in promoting pathophysiological conditions of vessel. We investigated the effects of NO donors on the COX-1 and COX-2 mRNA/protein expression, as well as the nitrite production in culture medium of vascular smooth muscle cell (VSMC). VSMC was primarily cultured from thoracic aorta of rat. In this experiments, COX-1 and COX-2 mRNA/protein expressions were analysed and nitrite productions were investigated using Griess reagent. VSMC did not express COX-2 protein in basal condition (Non-lipopolysaccharide (LPS) stimulated). In LPS-stimulated experiments, after 3 hours of NO donor pretreatment, LPS 10 microgram/ml was treated for 24 hours. COX-1 protein expressions were unchanged by SNP and NOR-3. NOR-3 significantly increased COX-2 mRNA/protein expression under LPS stimulation. In contrast, SNP did not increase COX-2 mRNA/protein expression under LPS stimulation. Nitrite production was higher in NOR-3 treatment than SNP treatment under LPS stimulation. These results suggest that the expression of COX-2 in VSMC is regulated by NOR-3, COX-2 expressions were depending on the types of NO donor and LPS stimulation in VSMC.
