Curcumin protects against the intestinal ischemia-reperfusion injury: involvement of the tight junction protein ZO-1 and TNF-alpha related mechanism.
10.4196/kjpp.2016.20.2.147
- Author:
Shuying TIAN
1
;
Ruixue GUO
;
Sichen WEI
;
Yu KONG
;
Xinliang WEI
;
Weiwei WANG
;
Xiaomeng SHI
;
Hongyu JIANG
Author Information
1. Department of Gastroenterology, Cangzhou Central Hospital, Hebei 061001, China. sichenwei11@163.com
- Publication Type:Original Article
- Keywords:
Curcumin;
Intestine;
Intestinal mucosa barrier;
Intestinal permeability;
Ischemia/reperfusion (I/R) injury;
Tight junction protein (ZO-1)
- MeSH:
Amine Oxidase (Copper-Containing);
Animals;
Blotting, Western;
Curcumin*;
Eosine Yellowish-(YS);
Immunohistochemistry;
Intestinal Mucosa;
Intestine, Small;
Intestines;
Permeability;
Rats, Wistar;
Reperfusion Injury*;
Tight Junctions*;
Tumor Necrosis Factor-alpha*;
Zonula Occludens-1 Protein*
- From:The Korean Journal of Physiology and Pharmacology
2016;20(2):147-152
- CountryRepublic of Korea
- Language:English
-
Abstract:
Present study aimed to investigate the eff ect of curcumin-pretreatment on intestinal I/R injury and on intestinal mucosa barrier. Thirty Wistar rats were randomly divided into: sham, I/R, and curcumin groups (n=10). Animals in curcumin group were pretreated with curcumin by gastric gavage (200 mg/kg) for 2 days before I/R. Small intestine tissues were prepared for Haematoxylin & Eosin (H&E) staining. Serum diamine oxidase (DAO) and tumor necrosis factor (TNF)-alpha levels were measured. Expression of intestinal TNF-alpha and tight junction protein (ZO-1) proteins was detected by Western blot and/or immunohistochemistry. Serum DAO level and serum and intestinal TNF-alpha leves were signifi cantly increased after I/R, and the values were markedly reduced by curcumin pretreatment although still higher than that of sham group (p<0.05 or p<0.001). H&E staining showed the significant injury to intestinal mucosa following I/R, and curcumin pretreatment signifi cantly improved the histological structure of intestinal mucosa. I/R insult also induced significantly down-regulated expression of ZO-1, and the eff ect was dramatically attenuated by curcumin-pretreatment. Curcumin may protect the intestine from I/R injury through restoration of the epithelial structure, promotion of the recovery of intestinal permeability, as well as enhancement of ZO-1 protein expression, and this eff ect may be partly attributed to the TNF-alpha related pathway.
