High glucose and palmitate increases bone morphogenic protein 4 expression in human endothelial cells.
10.4196/kjpp.2016.20.2.169
- Author:
Oak Kee HONG
1
;
Soon Jib YOO
;
Jang Won SON
;
Mee Kyoung KIM
;
Ki Hyun BAEK
;
Ki Ho SONG
;
Bong Yun CHA
;
Hanjoong JO
;
Hyuk Sang KWON
Author Information
1. Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea. drkwon@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Bone morphogenic protein 4;
Diabetes mellitus;
Free fatty acid;
High glucose
- MeSH:
Atherosclerosis;
Diabetes Mellitus;
Diabetic Angiopathies;
Endothelial Cells*;
Fatty Acids, Nonesterified;
Glucose*;
Human Umbilical Vein Endothelial Cells;
Humans*;
Hyperglycemia;
Reactive Oxygen Species;
RNA, Small Interfering;
Transfection
- From:The Korean Journal of Physiology and Pharmacology
2016;20(2):169-175
- CountryRepublic of Korea
- Language:English
-
Abstract:
Here, we investigated whether hyperglycemia and/or free fatty acids (palmitate, PAL) aff ect the expression level of bone morphogenic protein 4 (BMP4), a proatherogenic marker, in endothelial cells and the potential role of BMP4 in diabetic vascular complications. To measure BMP4 expression, human umbilical vein endothelial cells (HUVECs) were exposed to high glucose concentrations and/or PAL for 24 or 72 h, and the effects of these treatments on the expression levels of adhesion molecules and reactive oxygen species (ROS) were examined. BMP4 loss-of-function status was achieved via transfection of a BMP4-specific siRNA. High glucose levels increased BMP4 expression in HUVECs in a dose-dependent manner. PAL potentiated such expression. The levels of adhesion molecules and ROS production increased upon treatment with high glucose and/or PAL, but this eff ect was negated when BMP4 was knocked down via siRNA. Signaling of BMP4, a proinflammatory and pro-atherogenic cytokine marker, was increased by hyperglycemia and PAL. BMP4 induced the expression of infl ammatory adhesion molecules and ROS production. Our work suggests that BMP4 plays a role in atherogenesis induced by high glucose levels and/or PAL.
