Mitochondrial calcium uniporter inhibition attenuates mouse bone marrow-derived mast cell degranulation induced by beta-1,3-glucan.
10.4196/kjpp.2016.20.2.213
- Author:
Dang Van CUONG
1
;
Hyoung Kyu KIM
;
Jubert MARQUEZ
;
Nari KIM
;
Kyung Soo KO
;
Byoung Doo RHEE
;
Jin HAN
Author Information
1. National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Department of Health Sciences and Technology, BK21 Project Team, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University, Busan 47392, Korea. phyhanj@inje.ac.kr
- Publication Type:Original Article
- Keywords:
Beta-1,3-glucan;
Mast cell degranulation;
Mitochondrial calcium uniporter
- MeSH:
Animals;
Calcimycin;
Calcium*;
Cytosol;
Exocytosis;
Inflammation;
Ion Transport*;
Mast Cells*;
Membrane Potentials;
Mice*;
Mitochondria;
Peptidoglycan;
Ruthenium Red;
Shock
- From:The Korean Journal of Physiology and Pharmacology
2016;20(2):213-220
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mast cells are primary mediators of allergic inflammation. Beta-1,3-glucan (BG) protects against infection and shock by activating immune cells. Activation of the BG receptor induces an increase in intracellular Ca2+, which may induce exocytosis. However, little is known about the precise mechanisms underlying BG activation of immune cells and the possible role of mitochondria in this process. The present study examined whether BG induced mast cell degranulation, and evaluated the role of calcium transients during mast cell activation. Our investigation focused on the role of the mitochondrial calcium uniporter (MCU) in BG-induced degranulation. Black mouse (C57) bone marrow-derived mast cells were stimulated with 0.5 microg/ml BG, 100 microg/ml peptidoglycan (PGN), or 10 microM A23187 (calcium ionophore), and dynamic changes in cytosolic and mitochondrial calcium and membrane potential were monitored. BG-induced mast cell degranulation occurred in a time-dependent manner, and was significantly reduced under calcium-free conditions. Ruthenium red, a mitochondrial Ca2+ uniporter blocker, significantly reduced mast cell degranulation induced by BG, PGN, and A23187. These results suggest that the mitochondrial Ca2+ uniporter has an important regulatory role in BG-induced mast cell degranulation.
