Effects of prunetin on the proteolytic activity, secretion and gene expression of MMP-3 in vitro and production of MMP-3 in vivo.
10.4196/kjpp.2016.20.2.221
- Author:
Dae Cheol NAM
1
;
Bo Kun KIM
;
Hyun Jae LEE
;
Hyun Dae SHIN
;
Choong Jae LEE
;
Sun Chul HWANG
Author Information
1. Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea. hscspine@hanmail.net LCJ123@cnu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Chondrocyte;
Metalloproteinase;
Osteoarthritis;
Prunetin
- MeSH:
Animals;
Blotting, Western;
Caseins;
Chondrocytes;
Gene Expression*;
Interleukin-1beta;
Knee Joint;
Osteoarthritis;
Rats;
Thrombospondins
- From:The Korean Journal of Physiology and Pharmacology
2016;20(2):221-228
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated whether prunetin affects the proteolytic activity, secretion, and gene expression of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as in vivo production of MMP-3 in the rat knee joint to evaluate the potential chondroprotective eff ect of prunetin. Rabbit articular chondrocytes were cultured in a monolayer, and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure interleukin-1beta (IL-1beta)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and ADAMTS-5. In rabbit articular chondrocytes, the effects of prunetin on IL-1beta-induced secretion and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The eff ect of prunetin on MMP-3 protein production was also examined in vivo. The results were as follows: (1) prunetin inhibited the gene expression of MMP-3, MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5; (2) prunetin inhibited the secretion and proteolytic activity of MMP-3; (3) prunetin suppressed the production of MMP-3 protein in vivo. These results suggest that prunetin can regulate the gene expression, secretion, and proteolytic activity of MMP-3, by directly acting on articular chondrocytes.
